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Objective: To investigate the inhibitory effect of ethanol extract of Forsythia suspensa root (FSEER) on growth of esophageal cancer cells in nude mice. Methods: The inhibitory effect of FSEER on growth of esophageal cancer cell lines including TE-1, TE-13, Yes-2 and Eca-109 was measured by MTT method. The xenograft tumor models of TE-13 cells in nude mice were established and divided into two groups: treatment group [treated with FSEER (50 mg/kg)] and the control group (treated with equal volume of normal saline). Then the growth of xenograft tumors in nude mice was observed and their sizes and weights were measured. The apoptosis of tumor tissues in nude mice was deteced by TUNEL staining analysis. The expressions of Bcl-2 and Bax in tumor tissues were detected by immunohistochemical method. Morphological changes of lung and liver tissues in nude mice were observed by hematoxylin-eosin staining. Results: FSEER had obvious inhibitory effect on the growth of esophageal cancer cells in vitro (P < 0.01). Moreover, the growth of TE-13 cells was inhibited by FSEER in the dose-and timedependent manner (P < 0.01). The average volume and weight of tumor tissues in nude mice of FSEER treatment group were significantly smaller and lower, respectively, as compared with those of the control group (P < 0.05). Comparing to the control group, the number of apoptotic cells in FSEER treatment group was significantly increased (P < 0.05), while the expression level of Bcl-2 was decreased (P < 0.05), but Bax expression level was increased (P < 0.05). No obvious morphological changes were found in liver and lung tissues in FSEER treatment group and the control group. Conclusion: FSEER has inhibitory effect on the growth of xenograft tumor of esophageal cancer cells in nude mice, which may be related to inducing apoptosis.
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<p><b>OBJECTIVE</b>To analyze the value of directional preponderance (DP) in vestibular dynamic compensation evaluation.</p><p><b>METHODS</b>One hundred fifty-five patients with sudden deafness accompanied vertigo were asked to accept routine vestibular tests including the spontaneous nystagmus (SN) and caloric test with video-nystagmography. DP, SN and unilateral weakness (UW) were selected as the observation parameters. Then the correlation analysis between DP, SN, UW and course of disease were investigated respectively. The change characteristic of the three parameters was also analyzed in the course of vestibular compensation.</p><p><b>RESULTS</b>Of the 155 patients, there were negative correlations between SN, DP and course of disease (r(s) = -0.386, r(s) = -0.424, P < 0.05), positive correlation between SN and DP (r(s) = 0.543, P < 0.05), but no correlation between UW and course of disease (r(s) = 0.004, P > 0.05), and also positive correlation (r(s) = 0.274, P < 0.05) between DP and UW. According to three parameters were positive or negative, three main groups (DP+UW+SN+, DP+UW+SN- and DP-UW+SN-) in DP, UW, SN. The course of disease in three main groups was positively skewed distribution. Median was 6 days, 10 days and 20 days respectively. Through Kruskal-Wallis Test, chi(2) = 24.63, P < 0.05, there was statistical significance among the above three main groups.</p><p><b>CONCLUSIONS</b>DP keeps on changing in the acute stage, static and dynamic compensation stage of peripheral vestibular disease. This indicates that DP can be considered as an objective evaluation index of vestibular dynamic compensation and as a guidance of the vestibular rehabilitation evaluation.</p>
Subject(s)
Adolescent , Adult , Aged , Aged, 80 and over , Female , Humans , Middle Aged , Young Adult , Caloric Tests , Electronystagmography , Hearing Loss, Sudden , Diagnosis , Vertigo , Diagnosis , Vestibular Function TestsABSTRACT
Objective:To investigate the immune response after injecting polysaccharide nucleic acid of Bacillus Calmette-Guerin(BCG-PSN)under the mucous membrane of bladder in rabbits and to search for the most suitable dose of BCG-PSN.Methods:The rabbits were randomly divided into 5 groups:high dose(0.35 mg/ml)BCG-PSN group,mid- dle dose(0.175 mg/ml)BCG-PSN group,low dose(0.0875 mg/ml)BCG-PSN group,BCG(0.35 mg/ml)control group and BCG-PSN(0.35 mg/ml)intra-bladder perfusion control group.T lymphocyte subsets(CD4~+,CD8~+)in the peripheral blood were determined by flow cytometry before and 1 week,2 weeks,1 month and 3 months after the treatment; the levels of IL-2,TNF-?,and IFN-?of peripheral blood were determined by enzyme-linked immunosorbent assay;H-E and Masson staining was used for the pathological examination of the bladder.Results:(1)BCG-PSN injection increased the number of CD4~+ and CD8~+ T lymphocytes in a time- and dose-dependent manner,with the peak numbers appeared 2 weeks after BCG-PSN injection;the numbers restored to the normal levels 3 months after BCG-PSN injection.BCG control group had a similar changing pattern to the BCG-PSN injection group.The number of CD4~+ T cells BCG-PSN perfusion group was significantly lower than that in the high-dose BCG-PSN injection group(P